Invitrogen gateway manual

The easy-to-use choice for everyday cloning. Invitrogen Gateway recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system in just a few simple steps. For details about a particular Invitrogen destination vector or expression system, refer to the manual for the specific destination vector or system. All Gateway ® product manuals are available for downloading from our Web site (www.doorway.ru) or by contacting Technical Service (see page 52). Glossary of Terms ®. For more information on the Gateway® Technology, refer to the Gateway® Technology manual. This manual is available for downloading from our Web site (www.doorway.ru) or by contacting Technical Service (page 18). Experimental Outline To convert your vector to a destination vector, you will: 1. Choose the appropriate reading frame cassette to use.

TOPO TA Cloning—To create a Gateway entry clone. Step 1—Produce PCR product. Produce PCR products using Taq polymerase and your own protocol. End the PCR reaction with a final 7 to 30 minute extension step. Step 2—Perform the TOPO Cloning Reaction. Set up one of the following Invitrogen TOPO Cloning reactions using the reagents in the. Life Technologies, a Division of Invitrogen Corporation www.doorway.ru as described in the GATEWAY Cloning Technology Instruction Manual, but does not. specialized or customized vector, the Invitrogen™ Gateway™ Vector Conversion System can convert any vector into one compatible for Gateway cloning. Figure 1. Gateway technology facilitates cloning of genes into and back out of multiple vectors via site-specific recombination. Once a gene is cloned into an Entry clone, you can then.

The primer design tool will not add translation initiation motif sequences. Users will need to add these motifs manually as extensions during the primer design. Convert your own vector to a destination vector. For details about a particular Invitrogen destination vector or expression system, refer to the manual for the. Types of Products This manual is supplied with the following products listed below. Product For more information, refer to www.doorway.ru or.