Invitrogen generacer manual

 · First round PCR was performed using the GeneRacer 50 adapter primer and a gene specific primer designed from sequences of the initially isolated DD-PCR product (Dif6 FGR1 5′-RLM-RACE was performed according to the Invitrogen GeneRacer manual with modifications. GeneRacer® is an advanced RACE (rapid amplification of cDNA ends) technique that improves the efficiency of amplifying full-length 5´ and 3´ cDNA ends. With the GeneRacer® Kit you can: • Generate cDNA from transcripts up to 10 kb in length. • Obtain the full-length 5´ end of rare transcripts at fewer than 30 copies per cell. GeneRacer kit (Invitrogen) The GeneRacer kit provides a method to obtain full-length 5’ and 3’ ends of cDNA using known cDNA sequence. The kit ensures the amplification of only full-length transcripts via elimination of truncated messages formed during the amplification process.

GeneRacer® is an advanced RACE (rapid amplification of cDNA ends) technique that improves the efficiency of amplifying full-length 5´ and 3´ cDNA ends. With the GeneRacer® Kit you can: • Generate cDNA from transcripts up to 10 kb in length. • Obtain the full-length 5´ end of rare transcripts at fewer than 30 copies per cell. GeneRacer™ ™ Module The GeneRacer Module contains reagents for five PCR-ready cDNA synthesis reactions, one control cDNA reaction, and primers for 50 PCRs. Reagents are included for dephosphorylating RNA, removing the mRNA cap structure, ligating the GeneRacer™ RNA Oligo to the mRNA, and reverse transcribing the mRNA. 5-RLM-RACE was performed according to the Invitrogen GeneRacer manual with modifications. 2–8 µg of total RNA was ligated directly to ng GeneRacer RNA adaptor (5’-CGACUGGAGCACGAGGACACUGACAUGGACUGAAGGAGUAGAAA-3’) using T4 RNA ligase (5 units) for 1 h at 37°C.

Mar Manual inspection of the genomic sequence of the operon in out using GeneRacer kit (Invitrogen) and total RNA from M. tuberculosis. RESULTS 54 - 80 Gene Racer Kit Protocol for 5' end Amplification (RACER kit, Invitrogen) and then full-length genes were isolated by III RT module of. Apr Therefore. Invitrogen makes no warranty of any kind regarding the contents of any publications or documentation. If you discover an error in any.